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Volume 27, Issue 125 (November & December 2019)
J Adv Med Biomed Res 2019, 27(125): 16-24 |
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Rajabi M, Zareian P, Shaddel M, Karimi I. Histopathological Investigation of the Effect of Ozonated Water on Skin Ulcer Healing Process in Isolation Stress Mouse Model. J Adv Med Biomed Res 2019; 27 (125) :16-24
URL: http://journal.zums.ac.ir/article-1-5694-en.html
URL: http://journal.zums.ac.ir/article-1-5694-en.html
1- Dept. of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
2- Dept. of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran ,zareianj2011@yahoo.com
3- Dept. of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
4- Dept. of Pathology, Faculty of Veterinary, Shahrekord University, Shahrekord, Iran
2- Dept. of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran ,
3- Dept. of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
4- Dept. of Pathology, Faculty of Veterinary, Shahrekord University, Shahrekord, Iran
Abstract: (146274 Views)
Background and Objective: Skin ulcers and their slow healing are considered as one of the important medical aspects. Nowadays, various antibiotics and materials are used to accelerate the wound healing process. One of the methods taken in the field of wound healing is using ozone. On the other hand, one of the factors that hurt the speed of wound healing is stress. Therefore, the present study aimed to assess the effect of ozonated water on healing of skin ulcers in stressful conditions.
Materials & Methods: A total of 80 male mice were randomly divided into four groups: stress -no ozone, stress - ozone, no stress-no ozone, no stress- ozone group. Isolation stress was used (daily, 4 hours, for 14 days). The healing process was evaluated based on measuring the wound surface area in days 2, 6, 10, and 14 and histopathological data.
Results: Isolation stress by decreasing the fibroblast maturation and angiogenesis in the wound leads to a reduction in the speed of wound healing. Ozone enhanced the rate of tissue repair in both stress and stressless situations by decreasing inflammation and increasing the fibroblast maturation, amount of collagen tissue, rate of re-epithelialization, new vascularization, and granulation tissue size.
Conclusion: The results of this study implied that treatment with ozone could accelerate the wound healing process and decrease the harmful effects of stress conditions in healing process of animal model.
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✅ The results of this study implied that treatment with ozone could accelerate the wound healing process and decrease the harmful effects of stress conditions in healing process of animal model.
Type of Study: Original Research Article |
Subject:
Bionanotechnology
Received: 2019/06/25 | Accepted: 2020/04/13 | Published: 2019/09/12
Received: 2019/06/25 | Accepted: 2020/04/13 | Published: 2019/09/12
References
1. Enoch S, Leaper DJ. Basic science of wound healing. Surgery. 2005; 23(2):37-42. [DOI:10.1383/surg.23.2.37.60352]
2. Bartlett A, Sanders AJ, Ruge F, Harding KG, Jiang WG.Potential implications of interleukin-7 in chronic wound healing. Exp Ther Med. 2016;12(1):33-40. [DOI:10.3892/etm.2016.3263] [PMID] [PMCID]
3. King A, Balaji S, Le LD, Crombleholme TM, Keswani SG. Regenerative wound healing: The role of interleukin-10. Adv Wound Care (New Rochelle). 2014;3(4):315-23. [DOI:10.1089/wound.2013.0461] [PMID] [PMCID]
4. Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg. 2006; 117(7 Suppl): 12S-34S. [DOI:10.1097/01.prs.0000225430.42531.c2] [PMID]
5. Koivisto L, Heino J, Häkkinen L, Larjava H.Integrins in wound healing. Adv Wound Care (New Rochelle). 2014; 3(12):762-83. [DOI:10.1089/wound.2013.0436] [PMID] [PMCID]
6. Yukami T, Hasegawa M, Matsushita Y, et al.Endothelial selectins regulate skin wound healing in cooperation with L-selectin and ICAM-1.J Leukoc Biol. 2007; 82(3):519-31. [DOI:10.1189/jlb.0307152] [PMID]
7. Nagaoka T, Kaburagi Y, Hamaguchi Y. Delayed wound healing in the absence of intercellular adhesion molecule-1 or L-selectin expression. Am J Pathol. 2000;157(1):237-47. [DOI:10.1016/S0002-9440(10)64534-8]
8. Gouin JP, Kiecolt-Glaser JK. The impact of psychological stress on wound healing: methods and mechanisms. Crit Care Nurs Clin North Am. 2012; 24(2):201-13. [DOI:10.1016/j.ccell.2012.03.006] [PMID] [PMCID]
9. Christian LM, Graham JE, Padgett DA, Glaser R, Kiecolt-Glaser JK. Stress and wound healing. Neuroimmunomodulation. 2006; 13(5-6): 337-46. [DOI:10.1159/000104862] [PMID] [PMCID]
10. Joachim RA, Handjiski B, Blois SM, Hagen E, Paus R, Arck PC. Stress-induced neurogenic inflammation in murine skin skews dendritic cells towards maturation and migration: key role of intercellular adhesion molecule-1/leukocyte function-associated antigen interactions. Am J Pathol. 2008; 173(5):1379-88. [DOI:10.2353/ajpath.2008.080105] [PMID] [PMCID]
11. Shurin MR, Kusnecov A, Hamill E, Kaplan S, Rabin BS. Stress- induced alteration of polymorphonuclear leukocyte function in rats. Brain Behav Immun. 1994; 8(2):163-9. [DOI:10.1006/brbi.1994.1015] [PMID]
12. Fitzpatrick E, Holland OJ, Vanderlelie JJ. Ozone therapy for the treatment of chronic wounds: A systematic review. Int Wound J. 2018; 15(4):633-44. [DOI:10.1111/iwj.12907] [PMID]
13. Xiao W, Tang H, Wu M, et al. Ozone oil promotes wound healing by increasing the migration of fibroblasts via PI3K/Akt/mTOR signaling pathway. Biosci Rep. 2017; 37(6): BSR20170658. [DOI:10.1042/BSR20170658] [PMID] [PMCID]
14. Degli Agosti I, Ginelli E, Mazzacane B, et al. Effectiveness of a short-term treatment of oxygen- zone therapy into healing in posttraumatic wound. Case Rep Med. 2016; 2016:9528572. [DOI:10.1155/2016/9528572] [PMID] [PMCID]
15. Kim HS, Noh SU, Han YW, et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci. 2009; 24(3):368-74. [DOI:10.3346/jkms.2009.24.3.368] [PMID] [PMCID]
16. Zhang J, Guan M, Xie C, Luo X, Zhang Q, Xue Y. Increased growth factors play a role in wound healing promoted by noninvasive oxygen-ozonetherapy in diabetic patients with foot ulcers. Oxid Med Cell Longev. 2014; 2014: 273475. [DOI:10.1155/2014/273475] [PMID] [PMCID]
17. Borges GÁ, Elias ST, da Silva SM, et al. In vitro evaluation of wound healing and antimicrobial potential of ozone therapy. J Craniomaxillofac Surg. 2017; 45(3):364-70. [DOI:10.1016/j.jcms.2017.01.005] [PMID]
18. Abramov Y, Golden B, Sullivan M, et al. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen. 2007;15(1):80-6. [DOI:10.1111/j.1524-475X.2006.00188.x] [PMID]
19. Slominski AT, Zmijewski MA. Glucocorticoids inhibit wound healing: novel mechanism of action. J Invest Dermatol. 2017; 137(5):1012-14. [DOI:10.1016/j.jid.2017.01.024] [PMID] [PMCID]
20. Padgett DA, Marucha PT, Sheridan JF. Restraint stress slows cutaneous wound healing in mice. Brain Behav Immun. 1998; 12(1):64-73. [DOI:10.1006/brbi.1997.0512] [PMID]
21. Romana-Souza B, Otranto M, Almeida TF, Porto LC, Monte-Alto-Costa A. Stress-induced epinephrine levels compromise murine dermal fibroblast activity through β-adrenoceptors. Exp Dermatol. 2011;20(5):413-9. [DOI:10.1111/j.1600-0625.2010.01239.x] [PMID]
22. Mercado AM, Padgett DA, Sheridan JF, Marucha PT. Altered kinetics of IL-1α, IL-1β, and KGF-1 gene expression in early wounds of restrained mice. Brain Behav Immun. 2002. 16(2):150-62. [DOI:10.1006/brbi.2001.0623] [PMID]
23. Yun YR, Won JE, Jeon E, et al. Fibroblast growth factors: Biology, function, and application for tissue regeneration. J Tissue Eng. 2010; 2010: 218142. [DOI:10.4061/2010/218142] [PMID] [PMCID]
24. Newman AC, Nakatsu MN, Chou W, Gershon PD, Hughes CC. The requirement for fibroblasts in angiogenesis: fibroblast-derived matrix proteins are essential for endothelial cell lumen formation. Mol Biol Cell. 2011; 22(20): 3791-800. [DOI:10.1091/mbc.e11-05-0393] [PMID] [PMCID]
26. Presta M, Dell'Era P, Mitola S, Moroni E, Ronca R, Rusnati M. Fibroblast growth factor/fibroblast growth factor receptor system in angiogenesis. Cytokine Growth Factor Rev. 2005;16(2):159-178 [DOI:10.1016/j.cytogfr.2005.01.004] [PMID]
27. Azuma K, Mori T, Kawamoto K, et al. Anti-inflammatory effects of ozonated water in an experimental mouse model. Biomed Rep. 2014; 2(5):671-74. [DOI:10.3892/br.2014.290] [PMID] [PMCID]
28. Sagai M, Bocci V. Mechanisms of action involved in ozone therapy: Is healing induced via a mild oxidative stress? Med Gas Res. 2011; 1:29. [DOI:10.1186/2045-9912-1-29] [PMID] [PMCID]
29. Biçer Ş, Sayar İ, Gürsul C, et al. Use of ozone to treat ileostomy dermatitis in an experimental rat model. Med Sci Monit. 2016; 22: 757-65. [DOI:10.12659/MSM.897696] [PMID] [PMCID]
30. Valacchi G, Bocci V. Studies on the biological effects of ozone: Release of factors from human endothelial cells. Mediators Inflamm. 2000; 9 (6):271-6. [DOI:10.1080/09629350020027573] [PMID] [PMCID]
31. Srivastava KK, Kumar R. Stress, oxidative injury and disease. Indian J Clin Biochem. 2015; 30(1): 3-10. [DOI:10.1007/s12291-014-0441-5] [PMID] [PMCID]
32. Wu H, Li F, Wang S, et al. Ceria nanocrystals decorated mesoporous silica nanoparticle based ROS-scavenging tissue adhesive for highly efficient regenerative wound healing. Biomaterials. 2018. 151: 66-7732. Mittal M, Siddiqui MR , Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal. 2014;20 (7): 1126-67 [DOI:10.1016/j.biomaterials.2017.10.018] [PMID]
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