Volume 29, Issue 134 (May & June 2021)                   J Adv Med Biomed Res 2021, 29(134): 152-160 | Back to browse issues page

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Nuoroozi G, Omidi M, Rajabibazl M, Hoseinpoor R. Synergistic Effects of Graphene Oxide and Vascular Endothelial Growth Factor Immobilized in Polycaprolactone Nanofiber as a Candidate for Diabetic Wound Healing. J Adv Med Biomed Res 2021; 29 (134) :152-160
URL: http://journal.zums.ac.ir/article-1-6041-en.html
Synergistic Effects of Graphene Oxide and Vascular Endothelial Growth Factor Immobilized in Polycaprolactone Nanofiber as a Candidate for Diabetic Wound Healing
Ghader Nuoroozi1 , Meisam Omidi2 , Masoumeh Rajabibazl *3 , Reyhaneh Hoseinpoor4
1- Dept. of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Protein Research Center, Shahid Beheshti University, Tehran, Iran
3- Dept. of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , rajabibazl_m@yahoo.com
4- Dept. of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (135490 Views)

   Background & Objective: The combination of two or more therapeutic agents and their synergetic impacts can be therapeutically effective against multifactorial diseases, such as diabetic foot ulcers. This study demonstrates the application of a nanofiber-based drug delivery system with a controlled release of the growth factor. Various studies have shown that vascular endothelial growth factor (VEGF) stimulates angiogenesis via the VEGF signaling pathway and graphene oxide (GO) has been reported to possess antibacterial property. Therefore, VEGF and GO are hypothesized to have wound-healing effects when used synergistically.
 Materials & Methods:  In this study, VEGF was purified and verified by western blotting. GO and polycaprolactone (PCL) were prepared by electrospinning and were characterized by scanning electron microscope. Next, VEGF was immobilized by EDC/NHS linker in PCL-GO. Staphylococcus aureus and Escherichia coli were used to evaluate the antibacterial property of GO. Biodegradation and other release properties of the nanofibers were assessed. Moreover, the nanofibers were studied for cell viability and gene expression using human umbilical vein endothelial cells.
 Results:  The re-analysis of the protein-protein interaction network from the GO database confirmed the centrality of the nitric oxide synthase 3 (eNOS) showing its effects on the expression of other proteins. In addition, the PCL-GO nanofiber loaded with VEGF was studied for the expression of the eNOS gene in the VEGF signaling pathway. It was observed that PCL-GO-VEGF led to an increased expression of the eNOS gene in two weeks.
 Conclusion:   Based on the observed antibacterial property and angiogenesis influence, PCL-GO-VEGF can be considered as a candidate to promote diabetic wound healing.

 
 
Keywords: Graphene oxide, Nanofiber, Nitric oxide synthase type III, Polycaprolactone, VEGF-A
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✅ Based on the observed antibacterial property and angiogenesis influence, PCL-GO-VEGF can be considered as a candidate to promote diabetic wound healing.


Type of Study: Original Research Article | Subject: Bionanotechnology
Received: 2020/05/31 | Accepted: 2020/10/4 | Published: 2020/12/30
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