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Volume 32, Issue 150 (January & February 2024)
J Adv Med Biomed Res 2024, 32(150): 79-86 |
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Soltan- Dallal M M, Mirbagheri S Z, Vahedi S, Mohammadi M R, Molla Agha Mirzaei H. Evaluation of Multidrug Resistance and TEM and SHV Broad-Spectrum Beta-Lactamase Genes in Escherichia coli Obtained from Patients with Urinary Tract Infections in Alborz Province, Iran. J Adv Med Biomed Res 2024; 32 (150) :79-86
URL: http://journal.zums.ac.ir/article-1-7157-en.html
URL: http://journal.zums.ac.ir/article-1-7157-en.html
Mohammad Mehdi Soltan- Dallal 
1, Seyedeh Zohre Mirbagheri2 , Saeed Vahedi3 , Mohammad Reza Mohammadi4 , Hedroosha Molla Agha Mirzaei5
1, Seyedeh Zohre Mirbagheri2 , Saeed Vahedi3 , Mohammad Reza Mohammadi4 , Hedroosha Molla Agha Mirzaei5
1- Food Microbiology Research Center, Tehran University of Medical Sciences, Iran , msoltandallal@gmail.com
2- Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran
3- Instructor, Department of Anesthesiology, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5- Master of Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences,Tehran, Iran.
2- Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran
3- Instructor, Department of Anesthesiology, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5- Master of Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences,Tehran, Iran.
Abstract: (139 Views)
Background and Objective: Investigating multidrug resistance and TEM and SHV broad-spectrum beta-lactamase genes in Escherichia coli bacteria isolated from patients with urinary tract infections is very useful to improve the treatment of infection and prevent the failure of treatment of urinary tract infections.The aim of this study was to investigate multidrug resistance and TEM and SHV broad-spectrum beta-lactamase genes in Escherichia coli bacteria isolated from patients with urinary tract infections.
Materials and Methods: In this study, 188 strains of Escherichia coli, which cause urinary tract infections in Alborz province, were studied. Urine samples were cultured on EMB and Blood Agar media. Differential tests were performed for final identification. ESBL-producing strains were identified, PCR was performed to survey the abundance of ESBL-producing genes.
Results:Based on the results of the disk diffusion and Double-disk synergy testS, 82 (43.6%) strains were determined as the final producer of ESBL. out of these isolates, the frequency of SHV, TEM, and CTX genes measured 64.3%, 55.9%, and 21.4%, respectively. These results showed that 12 (14.28%) of Escherichia coli isolated have all genes, 26 (30.95%) had 2 genes and 36 (42.85%) had one gene.
Conclusion:According to the results, it was found that imipenem with the lowest resistance is the best drug in the treatment, and carbapenems are the best drug for treating diseases caused by Escherichia coli. The results of the current study may be useful in replacing ESBL enzyme resistance screening with more modern sensitivity measurement methods such as MIC and Etest.
Materials and Methods: In this study, 188 strains of Escherichia coli, which cause urinary tract infections in Alborz province, were studied. Urine samples were cultured on EMB and Blood Agar media. Differential tests were performed for final identification. ESBL-producing strains were identified, PCR was performed to survey the abundance of ESBL-producing genes.
Results:Based on the results of the disk diffusion and Double-disk synergy testS, 82 (43.6%) strains were determined as the final producer of ESBL. out of these isolates, the frequency of SHV, TEM, and CTX genes measured 64.3%, 55.9%, and 21.4%, respectively. These results showed that 12 (14.28%) of Escherichia coli isolated have all genes, 26 (30.95%) had 2 genes and 36 (42.85%) had one gene.
Conclusion:According to the results, it was found that imipenem with the lowest resistance is the best drug in the treatment, and carbapenems are the best drug for treating diseases caused by Escherichia coli. The results of the current study may be useful in replacing ESBL enzyme resistance screening with more modern sensitivity measurement methods such as MIC and Etest.
Keywords: Escherichia coli, Drug resistance, Beta-lactamases, Extended-spectrum beta-lactamases (ESBLs)
Type of Study: Original Article |
Subject:
Medical Biology
Received: 2022/12/27 | Accepted: 2023/11/1 | Published: 2024/01/30
Received: 2022/12/27 | Accepted: 2023/11/1 | Published: 2024/01/30
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