Mon, Jun 9, 2025
[Archive]
Volume 32, Issue 153 (July & August 2024)
J Adv Med Biomed Res 2024, 32(153): 288-298 |
Back to browse issues page
Ethics code: IR.KUMS.REC.1400.094
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Abdolmaleki A, Pazhouhi M, Rashidi I, Heshmati S, Jalili C, Khani-Hematabadi F. Molecular Basis of Apoptosis Induced by Taraxasterol on Human Melanoma Cell Line Growth Inhibition: An in-Vitro Study. J Adv Med Biomed Res 2024; 32 (153) :288-298
URL: http://journal.zums.ac.ir/article-1-7415-en.html
URL: http://journal.zums.ac.ir/article-1-7415-en.html
Amir Abdolmaleki1 
, Mona Pazhouhi2 , Iraj Rashidi2 , Saeid Heshmati2 , Cyrus Jalili2 , Fuzieh Khani-Hematabadi *3
, Mona Pazhouhi2 , Iraj Rashidi2 , Saeid Heshmati2 , Cyrus Jalili2 , Fuzieh Khani-Hematabadi *3
1- Department of Operating Room, Nahavand School of Allied Medical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Kermanshah university of medical sciences
3- Kermanshah university of medical sciences ,fuziekhani@yahoo.com
2- Kermanshah university of medical sciences
3- Kermanshah university of medical sciences ,
Abstract: (387 Views)
Background & Objective: Melanoma, one of the most lethal cancers, originates from epidermal layer. An advanced type of malignant melanoma represents a poor response to chemotherapy or other medications due to intrinsic and/or acquired resistance to antineoplastic drugs. Taraxasterol is a pentacyclic-triterpene agent mainly extracted from Dandelion herb with anti-proliferative and apoptotic features on cancer cells. Thus, this paper investigated the apoptosis pathway caused by Taraxasterol in the human melanoma cell line (hMCL).
Materials & Methods: hMCLs were treated with Taraxasterol and IC50 index was calculated using MTT assay. Then, apoptosis rate was evaluated by DNA Fragmentation Calorimetric technique. Finally, apoptosis pathway was investigated through various molecular laboratory assays.
Results: Low cellular viability level was found as concentration and time-dependent routes. Induction of apoptosis by IC50 value of Taraxasterol was found significantly (p<0.05) effective. Mitochondria membrane potential index was reduced by Taraxasterol significantly (p<0.05). Also, the cytosolic levels of cytochrome C and expression level of caspase 3, 8, and 9 genes in hMCL were increased significantly (p<0.05) following Taraxasterol administration.
Conclusion: Taraxasterol represents anti-proliferative and toxic effects against hMCL by induction of apoptosis.
Materials & Methods: hMCLs were treated with Taraxasterol and IC50 index was calculated using MTT assay. Then, apoptosis rate was evaluated by DNA Fragmentation Calorimetric technique. Finally, apoptosis pathway was investigated through various molecular laboratory assays.
Results: Low cellular viability level was found as concentration and time-dependent routes. Induction of apoptosis by IC50 value of Taraxasterol was found significantly (p<0.05) effective. Mitochondria membrane potential index was reduced by Taraxasterol significantly (p<0.05). Also, the cytosolic levels of cytochrome C and expression level of caspase 3, 8, and 9 genes in hMCL were increased significantly (p<0.05) following Taraxasterol administration.
Conclusion: Taraxasterol represents anti-proliferative and toxic effects against hMCL by induction of apoptosis.
Type of Study: Original Research Article |
Subject:
Life Science
Received: 2023/12/31 | Accepted: 2024/11/4 | Published: 2024/08/20
Received: 2023/12/31 | Accepted: 2024/11/4 | Published: 2024/08/20
References
1. Asgari R, Bidmeshkipour A, Mansouri K, Bakhtiari M, Mozafari H, Abdolmaleki A. Fas cell surface death receptor/Fas ligand genetic variants in gastric cancer patients: A case-control study. Indian J Med Res. 2022 ;156(1):77-82. [DOI:10.4103/ijmr.IJMR_2058_19] [PMID] [PMCID]
2. Ernst AK, Putscher A, Samatov TR, Suling A, Galatenko VV, Shkurnikov MY,et al. Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy. PloS one. 2018 ;13(2):e0192525. [DOI:10.1371/journal.pone.0192525] [PMID] [PMCID]
3. Bandarchi B, Ma L, Navab R, Seth A, Rasty G. From melanocyte to metastatic malignant melanoma. Dermatol Res Pract. 2010;2010(1):583748. [DOI:10.1155/2010/583748] [PMID] [PMCID]
4. Karimi SA, Abdolmaleki A, Sadeghi A, Naseri S, Ayni M, Gholami MR. Drug delivery system through alginate dermal scaffold loaded with hydroalcoholic extract of Daphne Mucronata improves dermal excisional wound healing: An experimental research. Regenerat Engineer Translat Med. 2022 ;8(4):523-34. [DOI:10.1007/s40883-022-00252-3]
5. Rolnik A, Olas B. The plants of the Asteraceae family as agents in the protection of human health. Int J Molec Sci. 2021 ;22(6):3009. [DOI:10.3390/ijms22063009] [PMID] [PMCID]
6. Fan M, Zhang X, Song H, Zhang Y. Dandelion (Taraxacum Genus): A review of chemical constituents and pharmacological effects. Molecules. 2023;28(13):5022. [DOI:10.3390/molecules28135022] [PMID] [PMCID]
7. Bao T, Ke Y, Wang Y, Wang W, Li Y, Wang Y,et al. Taraxasterol suppresses the growth of human liver cancer by upregulating Hint1 expression. J Molec Med. 2018;96:661-72. [DOI:10.1007/s00109-018-1652-7] [PMID]
8. Cario M, Taieb A. Isolation and culture of epidermal melanocytes. Skin Tissue Engineering: Method Protocol. 2019:33-46. [DOI:10.1007/978-1-4939-9473-1_3] [PMID]
9. Kamiloglu S, Sari G, Ozdal T, Capanoglu E. Guidelines for cell viability assays. Food Front. 2020;1(3):332-49. [DOI:10.1002/fft2.44]
10. Niksic H, Becic F, Koric E, Gusic I, Omeragic E, Muratovic S,et al. Cytotoxicity screening of thymus vulgaris L. essential oil in brine shrimp nauplii and cancer cell lines. Sci Rep. 2021 ;11(1):13178. [DOI:10.1038/s41598-021-92679-x] [PMID] [PMCID]
11. Ghanbari A, Jalili C, Abdolmaleki A, Shokri V. Effects of cisplatin and acacetin on total antioxidant status, apoptosis and expression of OCTN3 in mouse testis. Biotech Histochem. 2022;97(3):185-91. [DOI:10.1080/10520295.2021.1925347] [PMID]
12. Momcilovic M, Jones A, Bailey ST, Waldmann CM, Li R, Lee JT,et al. In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. Nature. 2019;575(7782):380-4. [DOI:10.1038/s41586-019-1715-0] [PMID] [PMCID]
13. Kalkavan H, Chen MJ, Crawford JC, Quarato G, Fitzgerald P, Tait SW,et al. Sublethal cytochrome c release generates drug-tolerant persister cells. Cell. 2022;185(18):3356-74. [DOI:10.1016/j.cell.2022.07.025] [PMID] [PMCID]
14. Harrison DC, Medhurst AD, Bond BC, Campbell CA, Davis RP, Philpott KL. The use of quantitative RT-PCR to measure mRNA expression in a rat model of focal ischemia-caspase-3 as a case study. Molec Brain Res. 2000;75(1):143-9. [DOI:10.1016/S0169-328X(99)00305-8] [PMID]
15. Bashir I, Dilshad E. A comparative study of Mentha longifolia var. asiatica and Zygophyllum arabicum ZnO nanoparticles against breast cancer targeting Rab22A gene. Plos one. 2024;19(8):e0308982. [DOI:10.1371/journal.pone.0308982] [PMID] [PMCID]
16. Li Z, Guo D, Yin X, Ding S, Shen M, Zhang R, Wang Y, Xu R. Zinc oxide nanoparticles induce human multiple myeloma cell death via reactive oxygen species and Cyt-C/Apaf-1/Caspase-9/Caspase-3 signaling pathway in vitro. Biomed Pharmacother. 2020;122:109712. [DOI:10.1016/j.biopha.2019.109712] [PMID]
17. Roshankhah S, Abdolmaleki A, Jalili C, Salahshoor MR. Crocin reduces oxidative stress produced by busulfan administration on fertility of male rats. J Adv Med Biomed Res. 2019;27(125):25-33. [DOI:10.30699/jambs.27.125.25]
18. Kondori BJ, Abdolmaleki A, Raei M, Alvanegh AG, Ghaleh HE. Intraperitoneal injection of mesenchymal stem cells-conditioned media (MSCS-CM) treated monocyte can potentially alleviate motor defects in experimental autoimmune encephalomyelitis female mice; An original experimental study. Transplant Immunol. 2024 3:102067. [DOI:10.1016/j.trim.2024.102067] [PMID]
19. Fulga A, Todiras M, Gudumac V, Tagadiuc O. Taraxacum officinale leaves and roots suppress glioma cell viability. J Biosci Med. 2022 ;10(3):175-89. [DOI:10.4236/jbm.2022.103017]
20. Hu C, Kitts DD. Antioxidant, prooxidant, and cytotoxic activities of solvent-fractionated dandelion (Taraxacum officinale) flower extracts in vitro. J Agri Food Chem. 2003;51(1):301-10. [DOI:10.1021/jf0258858] [PMID]
21. Suska F, Esposito M, Gretzer C, Källtorp M, Tengvall P, Thomsen P. IL-1α, IL-1β and TNF-α secretion during in vivo/ex vivo cellular interactions with titanium and copper. Biomaterials. 2003;24(3):461-8. [DOI:10.1016/S0142-9612(02)00359-9] [PMID]
22. Asgari R, Mansouri K, Abdolmaleki A, Bakhtiari M. Association of matrix metalloproteinases with male reproductive functions; with focus on MMP2, 7, and 9. Meta Gene. 2021;29:100906. [DOI:10.1016/j.mgene.2021.100906]
23. Ghanbari A, Jalili C, Abdolmaleki A, Zhaleh M, Zarinkhat A, Akhshi N. Falcaria vulgaris L. hydroalcoholic extract protects against harmful effects of mercuric chloride on the rat kidney. Avicenna J Phytomed. 2023;13(4):442.
24. Song Z, Wang J, Su Q, Luan M, Chen X, Xu X. The role of MMP-2 and MMP-9 in the metastasis and development of hypopharyngeal carcinoma. Brazil J Otorhinolaryngol. 2021 Sep 29;87:521-8 [DOI:10.1016/j.bjorl.2019.10.009] [PMID] [PMCID]
25. Pouremamali F, Vahedian V, Hassani N, Mirzaei S, Pouremamali A, Kazemzadeh H,et al. The role of SOX family in cancer stem cell maintenance: With a focus on SOX2. Pathol Res Pract. 2022;231:153783. [DOI:10.1016/j.prp.2022.153783] [PMID]
26. Yang, S., et al., SOX2 promotes tumorigenicity and inhibits the differentiation of I-type neuroblastoma cells. Int J Oncol. 2015. 46(1): 317-32. [DOI:10.3892/ijo.2014.2713] [PMID]
27. Fulda S, Wick W, Weller M, Debatin KM. Smac agonists sensitize for Apo2L/TRAIL-or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo. Nature Med. 2002;8(8):808-15. [DOI:10.1038/nm735] [PMID]
28. Dimberg LY, Anderson CK, Camidge R, Behbakht K, Thorburn A, Ford HL. On the TRAIL to successful cancer therapy? Predicting and counteracting resistance against TRAIL-based therapeutics. Oncogene. 2013;32(11):1341-50. [DOI:10.1038/onc.2012.164] [PMID] [PMCID]
29. Hiebl B, Peters S, Gemeinhardt O, Niehues SM, Jung F. Impact of serum in cell culture media on in vitro lactate dehydrogenase (LDH) release determination. J Cell Biotechnol. 2017;3(1):9-13 [DOI:10.3233/JCB-179002]
30. Forkasiewicz A, Dorociak M, Stach K, Szelachowski P, Tabola R, Augoff K. The usefulness of lactate dehydrogenase measurements in current oncological practice. Cell Molec Biol Lett. 2020;25:1-4. [DOI:10.1186/s11658-020-00228-7] [PMID] [PMCID]
31. Ghanbari A, Jalili C, Abdolmaleki A, Shokri V. Effects of cisplatin and acacetin on total antioxidant status, apoptosis and expression of OCTN3 in mouse testis. Biotech Histochem. 2022 ;97(3):185-91. [DOI:10.1080/10520295.2021.1925347] [PMID]
32. Lin CJ, Liu ST, Yang RC, Wang LH, Tsai CC, Chen TW,et al. Anticancer effects of Taraxacum via cell cycle arrest, necrosis, apoptosis, and endoplasmic reticulum stress. Am J Chinese Med. 2022 ;50(02):569-87. [DOI:10.1142/S0192415X22500227] [PMID]
33. Schug ZT, Gottlieb E. Cardiolipin acts as a mitochondrial signalling platform to launch apoptosis. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2009 ;1788(10):2022-31. [DOI:10.1016/j.bbamem.2009.05.004] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
