Background and Objective: Acne rosacea is a chronic skin disease with unknown etiology. Some reports have suggested an increased prevalence of helicobacter (H) pylori infection in patients with acne rosacea but it is controversial. This study was designed to compare the prevalence of H pylori between the acne rosacea patients and the healthy control group. Materials and Methods: This case-control study was performed on 30 patients with acne rosacea and 60 healthy individuals as a control group. 5 mL blood was taken from each person and anti-H pylori IgG was measured by enzyme-linked immunosorbent assay. Results: Mean age was 45.8±14.05 years in acne rosacea patients and 41.4±12.3 years in control group. Seventeen (56.7%) of patients and fifty two (86.2%) of controls were infected by H pylori (P=0.002), but mean serum anti-H pylori IgG level was significantly higher in acne rosacea patients comparing to healthy control group (139.49±174.15 g/mL vs 38.87±23.50, P=0.005). Conclusion: Despite increased mean serum anti-H pylori IgG levels in acne rosacea patients, H pylori was more prevalent in healthy control group, in this study.