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Nsonwu-Anyanwu A C, Eworo R E, Nsonwu M C, Akpan U O, Kalu F A, Nwevo E O. Age Related Changes in Indices of Bone Metabolism and Oxidative Stress in Postmenopausal Women in Southern Nigeria. J Adv Med Biomed Res 2023; 31 (145) :125-132
URL: http://journal.zums.ac.ir/article-1-6828-en.html
Age Related Changes in Indices of Bone Metabolism and Oxidative Stress in Postmenopausal Women in Southern Nigeria
Augusta Chinyere Nsonwu-Anyanwu * 1, Raymond Ekong Eworo2 , Magnus Chinonye Nsonwu3 , Uwem Okon Akpan2 , Faith Adamma Kalu2 , Eberechi Ogbonna Nwevo2
1- Dept. of Clinical Chemistry & Immunology, University of Calabar, Nigeria , austadechic@yahoo.com
2- Dept. of Clinical Chemistry & Immunology, University of Calabar, Nigeria
3- Dept. of Optometry, Imo State University, Owerri, Nigeria
Abstract:   (11127 Views)

Background and Objective: Oxidative stress (OS) associated with estrogen deficiency in menopause has been implicated in various complications of menopause. Some indices of OS, bone metabolism and uric acid (UA) in postmenopausal women were assessed as possible predictors of gouty osteoarthritis.
Materials and Methods: This case-control study enrolled 40 postmenopausal women and 60 premenopausal women as participants. The levels of reduced glutathione (GSH), nitric oxide (NO), total plasma peroxides (TPP), total antioxidant capacity (TAC), malondialdehyde (MDA), inorganic phosphate (Pi), total calcium (tCa) and UA were estimated by colorimetry, estradiol (E2) by ELISA and oxidative stress index (OSI) by calculation. Data were analyzed using t-test, correlation and regression at P<0.05.
Results: Postmenopausal women had higher UA, OSI and lipid peroxidation (higher MDA, TPP) with lower E2, tCa and antioxidants (reduced GSH, NO, TAC) compared to premenopausal women (P<0.05). Aging correlated negatively with E2 (r=-0.273, P=0.006), TAC (r=-0.484, P=<0.001), GSH (r=-0.306, P=0.002), NO (r=-0.337, P=0.001), tCa (r=-0.571, P=<0.001) and positively with TPP (r=0.445, P=<0.001), OSI (r=0.454, P=<0.001), MDA (r=0.505, P=<0.001) and UA (r=0.441, P=<0.001) in all women studied irrespective of menopause status. There were no associations between UA, tCa, Pi, E2 and indices of oxidative stress (TPP, TAC, OSI, MDA, GSH, NO) with menopause (R2 =0.216, P=0.728).
Conclusion: Elevated UA, lipid peroxidation and oxidative stress and reduced tCa, E2 and antioxidants observed in postmenopausal women may be associated with aging and not the menopausal status suggesting that their assessment may be utilized in predicting women at increased risk of gouty osteoarthritis.

Keywords: Menopause, Lipid peroxidation, Oxidative stress, Bone metabolism
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✅Elevated UA, lipid peroxidation and oxidative stress and reduced tCa, E2 and antioxidants observed in postmenopausal women may be associated with aging and not the menopausal status suggesting that their assessment may be utilized in predicting women at increased risk of gouty osteoarthritis.


Type of Study: Original Article | Subject: Life science
Received: 2022/01/19 | Accepted: 2022/11/21 | Published: 2023/03/13
References
1. Amrita J, Mahajan M, Bhanwer AJS, Mohan G. Oxidative stress: An effective prognostic tool for an early detection of cardiovascular disease in menopausal women. Biochem Res Inter. 2016; 2016: 1-7. [DOI:10.1155/2016/6157605] [PMID] [PMCID]
2. 2 Mohamad NV, Ima-Nirwana S, Chin KY. Are oxidative stress and inflammation mediators of bone loss due to estrogen deficiency? A review of current evidence. Endocr Metab Immune Disord Drug Targets. 2020;20(9):1478-1487. [DOI:10.2174/1871530320666200604160614] [PMID] [PMCID]
3. 3 Bonaccorsi G, Piva I, Greco P, Cervellati C. Oxidative stress as a possible pathogenic cofactor of post-menopausal osteoporosis: Existing evidence in support of the axis estrogen deficiency-redox imbalance-bone loss. Indian J Med Res.2018;47: 341-351. [DOI:10.4103/ijmr.IJMR_524_18] [PMID] [PMCID]
4. 4 Zhao F, Guo L, Wang X, Zhang Y. Correlation of oxidative stress-related biomarkers with postmenopausal osteoporosis: a systematic review and meta-analysis. Arch Osteoporos. 2021;16(1):4. [DOI:10.1007/s11657-020-00854-w] [PMID]
5. 5 Daniel WW, Cross CL. Biostatistics: a foundation for analysis in the health sciences. 10th ed. New York: John Wiley & Sons; 2013. Pp 189.
6. 6 Dehlin M, Jacobsson L, Roddy E. Global epidemiology of gout: prevalence, incidence, treatment patterns and risk factors. Nat Rev Rheumatol. 2020;16(7):380-390. [DOI:10.1038/s41584-020-0441-1] [PMID]
7. 7 Harma M, Erel O. Increased oxidative stress in patients with hydratiform mole. Swiss Med Wkly. 2003; 133: 563-566. [DOI:10.4414/smw.2003.10397] [PMID]
8. 8 Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for measurement of antioxidant activity in humans. J Clin Pathol 2001; 54(5): 356-361. [DOI:10.1136/jcp.54.5.356] [PMID] [PMCID]
9. 9 Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52:302- 10. [DOI:10.1016/S0076-6879(78)52032-6] [PMID]
10. 10 Bulaj G, Kortemme T, Goldenberg DP. Determination of sulfhydryl groups. Biochem. 1998; 7:8965-72. [DOI:10.1021/bi973101r] [PMID]
11. 11 Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5(1):62-71. [DOI:10.1006/niox.2000.0319] [PMID]
12. 12 Young DS. Phosphorus Metabolism. Clin. Chem. 1975; 21:342.
13. 13 Morin LG. Direct colorimetric determination of serum calcium in with O-cresolphthalein complexon. Am J Clin Path. 1974; 61(1):114-117. [DOI:10.1093/ajcp/61.1.114] [PMID]
14. 14 Town MH, Gehm S, Hammer B. A sensitive colorimetric method for the enzymatic determination of uric acid. J Clin. 1985; 23: 591.
15. 15 Sadeh D, Sela E, Hexter CS. Novel enzyme immunoassay for 17β-estradiol. J Immunol Methods 1979; 28(1-2): 125-31. [DOI:10.1016/0022-1759(79)90334-X] [PMID]
16. 16 Pardhe BD, Pathak S, Bhetwal A, et al .Effect of age and estrogen on biochemical markers of bone turnover in postmenopausal women: a population-based study from Nepal. Inter J Women's Health. 2017:9 781-788. [DOI:10.2147/IJWH.S145191] [PMID] [PMCID]
17. 17 Kilim SR, Chandala SR. A comparative study of lipid profile and oestradiol in pre- and post-menopausal women. J Clin Diagnos Res. 2013;7(8):1596. [DOI:10.7860/JCDR/2013/6162.3234] [PMID] [PMCID]
18. 18 Hyvärinen M, Juppi, HK, Taskinen S, et al. Metabolic health, menopause, and physical activity a 4-year follow-up study. Int J Obes 2022; 46: 544-554. [DOI:10.1038/s41366-021-01022-x] [PMID] [PMCID]
19. 19 El Habachi NM, Maklad HM, Sharara GM, Allam EA, Fawzy EM. A comparative study between the effect of 17-b estradiol and antioxidants combination on some menopausal changes in oophorectomised rats. Middle East Fert Soc J. 2014;19: 303-313. [DOI:10.1016/j.mefs.2014.02.003]
20. 20 Darawsha A, Trachtenberg A, Levy J, Sharoni Y. The protective effect of carotenoids, polyphenols, and estradiol on dermal fibroblasts under oxidative stress. Antioxidants. 2021; 10(12):2023. [DOI:10.3390/antiox10122023] [PMID] [PMCID]
21. 21 Bellanti F, Matteo M, Rollo T. Sex hormones modulate circulating antioxidant enzymes: Impact of estrogen therapy. Redox Biol. 2013; 1: 340-346 [DOI:10.1016/j.redox.2013.05.003] [PMID] [PMCID]
22. 22 Kypreos KE, Zafirovic S, Petropoulou PI, et al. Regulation of endothelial nitric oxide synthase and high-density lipoprotein quality by estradiol in cardiovascular pathology. J Cardiovasc Pharmacol Ther. 2014;19(3):256-268. [DOI:10.1177/1074248413513499] [PMID]
23. 23 Dahiya K, Dalal D, Malhotra V. Is nitric oxide level affected in postmenopausal women with hypothyroidism? World J Obstet Gynecol. 2017; 6(3): 16-20. [DOI:10.5317/wjog.v6.i3.16]
24. 24 Strehlow K, Rotter S, Wassmann S, et al. Modulation of antioxidant enzyme expression and function by estrogen. Circ Res. 2003; 93:170-177. [DOI:10.1161/01.RES.0000082334.17947.11] [PMID]
25. 25 Lean JM, Davies JT, Karen Fuller, et al . A crucial role for thiol antioxidants in estrogen-deficiency bone loss J. Clin. Invest. 112:915-923 (2003). [DOI:10.1172/JCI200318859] [PMID] [PMCID]
26. 26 Sánchez-Rodríguez MA, Zacarías-Flores M, Arronte-Rosales A, Correa-Muñoz E, Mendoza-Núñez VM. Menopause as risk factor for oxidative stress. Menopause. 2012;19: 361-7. [DOI:10.1097/gme.0b013e318229977d] [PMID]
27. 27 Thibodeau PA, Kachadourian R, Lemaya R, Bisson M, Dayb BJ, Paquette B. In vitro pro- and antioxidant properties of estrogens. J Steroid Biochem Mol Biol 2002; 81: 227-236. [DOI:10.1016/S0960-0760(02)00067-5] [PMID]
28. 28 Zhou Q, Zhu L, Zhang D, et al. Oxidative stress-related biomarkers in postmenopausal osteoporosis: A systematic review and meta-analyses. Dis Markers. 2016; 2016: 1-12. [DOI:10.1155/2016/7067984] [PMID] [PMCID]
29. 29 Maruhashi T, Nakashima A, Soga J, et al. Hyperuricemia is independently associated with endothelial dysfunction in postmenopausal women but not in premenopausal women. BMJ Open. 2013;3: e003659. [DOI:10.1136/bmjopen-2013-003659] [PMID] [PMCID]
30. 30 Hak EA, Curhan GC, Grodstein F, Choi HK. Menopause, postmenopausal hormone use and risk of incident gout. Ann Rheum Dis. 2010; 69(7): 1305-1309. [DOI:10.1136/ard.2009.109884] [PMID] [PMCID]
31. 31 Hak AL, Choi HK. Menopause, postmenopausal hormone use and serum uric acid levels in US women. The third national health and nutrition examination survey. Arithritis Res Therapy. 2008;10 (5): 116-22. [DOI:10.1186/ar2519] [PMID] [PMCID]
32. 32 Sonu Y, Avinash SS, Sreekantha K, Kumar A, Malathi A, Shivashankara R. Effect of estrogen on altering the serum and urinary levels of calcium, phosphate and magnesium in hysterectomised women compared to natural menopausal South Indian women: A case control study. Ind J Clin Biochem. 2016; 31(3):326-31 [DOI:10.1007/s12291-015-0532-y] [PMID] [PMCID]
33. 33 Noirrit-Esclassan E, Valera MC, Tremollieres F,et al. Critical role of estrogens on bone homeostasis in both male and female: From physiology to medical implications. Int J Mol Sci. 2021; 22:1568. [DOI:10.3390/ijms22041568] [PMID] [PMCID]
34. 34 Usoro CAO, Onyeukwu CU, Nsonwu AC. Biochemical bone turnover markers in postmenopausal women in Calabar municipality. Asian J Biochem. 2007; 2(2): 130-37. [DOI:10.3923/ajb.2007.130.135]
35. 35 Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018; 13: 757-72. [DOI:10.2147/CIA.S158513] [PMID] [PMCID]
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