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Volume 10, Issue 39 (6-2002)
J Adv Med Biomed Res 2002, 10(39): 5-12 |
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Esmaeil zadeh A A, Esfandiari N, Pakzad P. Comparison Between Elisa And Mixed Antiglobulin Reaction Methods For Antisperm Antibody Detection In Infertile Couples. J Adv Med Biomed Res 2002; 10 (39) :5-12
URL: http://journal.zums.ac.ir/article-1-407-en.html
URL: http://journal.zums.ac.ir/article-1-407-en.html
Abstract: (150835 Views)
Background: Considering the importance of immunologic infertility and also the existence of various screening methods of antisperm antibodies, this study was conducted to evaluate ELISA (Enzyme Linked lmmunosorbent Assey) and MAR (Mixed Antiglobulin Reaction) methods in people referring to the hospitals affiliated to Shahid Beheshti University of Medical Sciences.
Materials and Methods: This study was a comparison of laboratory methods which was carried out on 20 fertile and 20 infertile couples. The serum samples collected were studied to determine the level of antisperm antibodies using Agglutination (as standard method), ELISA, and MAR. The data obtained were then analyzed by Fisher exact test and Chi-square for sensitivity, specificity, PPV, and NPV.
Results: The frequency of antisperm antibodies in infertile people was 5/2 times more than that of fertile people (P<0.005), and the positive results in the females was 2.5 times more than the males. Age and cigarette smoking were found not to be related to antisperm antibody. Specificity, sensitivity, PPV, and NPV were 98% 16%, 86%, and 57% respectively for MAR and 88%, 13.5%,50%, and 54% for ELISA.
Conclusion: MAR and ELISA methods are of less sensitivity but appropriate specificity for diagnosis of antisperm antibodies, but the combination of two or three methods can result in better immunoinfertility diagnosis. More investigations with greater number of samples are highly recommended.
Materials and Methods: This study was a comparison of laboratory methods which was carried out on 20 fertile and 20 infertile couples. The serum samples collected were studied to determine the level of antisperm antibodies using Agglutination (as standard method), ELISA, and MAR. The data obtained were then analyzed by Fisher exact test and Chi-square for sensitivity, specificity, PPV, and NPV.
Results: The frequency of antisperm antibodies in infertile people was 5/2 times more than that of fertile people (P<0.005), and the positive results in the females was 2.5 times more than the males. Age and cigarette smoking were found not to be related to antisperm antibody. Specificity, sensitivity, PPV, and NPV were 98% 16%, 86%, and 57% respectively for MAR and 88%, 13.5%,50%, and 54% for ELISA.
Conclusion: MAR and ELISA methods are of less sensitivity but appropriate specificity for diagnosis of antisperm antibodies, but the combination of two or three methods can result in better immunoinfertility diagnosis. More investigations with greater number of samples are highly recommended.
Type of Study: Original Research Article |
Received: 2008/11/10 | Accepted: 2002/06/15 | Published: 2002/06/15
Received: 2008/11/10 | Accepted: 2002/06/15 | Published: 2002/06/15
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