Volume 26, Issue 114 (3-2018)                   J Adv Med Biomed Res 2018, 26(114): 60-70 | Back to browse issues page

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Kazemizadeh A, Salouti M, Ahangari A, Shajari N, Aghayari M, Safari Zanjani L. Ceftazidime Loading on Ethylenediaminetetraacetic acid (EDTA): Release Kinetic and Enhanced Antimicrobial Effect on Pseudomonas aeruginosa. J Adv Med Biomed Res 2018; 26 (114) :60-70
URL: http://journal.zums.ac.ir/article-1-4961-en.html
Ceftazidime Loading on Ethylenediaminetetraacetic acid (EDTA): Release Kinetic and Enhanced Antimicrobial Effect on Pseudomonas aeruginosa
Alireza Kazemizadeh * 1, Mojtaba Salouti2 , Azam Ahangari3 , Nahid Shajari4 , Masoumeh Aghayari3 , Lili Safari Zanjani5
1- Dept. of Chemistry, Faculty of Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran , alirezakazemizadeh@yahoo.com
2- Biology Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran
3- Young Researchers and Elite Club, Islamic Azad University, Hidaj Branch, Hidaj, Iran
4- Dept. of Chemistry, Faculty of Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran
5- Dept. of Microbiology, Faculty of Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran
Abstract:   (150557 Views)

Background and Objective: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections. This study was planned to produce a new antimicrobial complex (ceftazidime loaded EDTA) and assess its release kinetic and antimicrobial effects on P. aeruginosa.
Materials and Methods: The loading of ceftazidime on EDTA was performed and confirmed by FT-IR spectroscopy. The evaluation of drug release was performed by using dialyze method at 0.5, 1, 2, 3, 4, 6, 8, 12, 24, 48, 72 and 96 h after ceftazidime loading on EDTA. The antimicrobial effect of the new drug on P. aeruginosa was investigated by macrodilution and well diffusion methods.
Results: FT-IR spectroscopy demonstrated ceftazidime loading on EDTA about 26.01 mol%. Evaluation of ceftazidime release indicated a gradual rise in drug release up to 8 h reaching 96% after 72 h. Well diffusion assay demonstrated an enhanced antimicrobial effect of ceftazidime after loading on EDTA in comparison with ceftazidime alone. MIC and MBC of ceftazidime alone determined 2 and 4 µg/ml, respectively.  MIC of the new antimicrobial complex was 0.5 µg/ml and its MBC was 1 µg/ml signifying the enhancement effect of EDTA on antibacterial activity of ceftazidime.
Conclusion: Our results showed that EDTA is potentially advantageous in controlling the release of ceftazidime as well as enhancing its antimicrobial effect against P. aeruginosa.

Keywords: Pseudomonas aeruginosa, Ceftazidime, EDTA, Controlled release, Antimicrobial effect
Full-Text [PDF 3149 kb]   (157486 Downloads)    
Type of Study: Clinical Trials |
Received: 2017/12/17 | Accepted: 2017/12/17 | Published: 2017/12/17
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