Volume 27, Issue 122 (May & June 2019)                   J Adv Med Biomed Res 2019, 27(122): 17-22 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Derakhshanfar A, Hashemi S, Moayedi J, Vahedi M, Mehrabani D, Valizadeh A. A Study on the Effects of Perovskia abrotanoides Karel on Experimental Skin Burn in Male Rat: in-vivo and in-vitro Findings. J Adv Med Biomed Res 2019; 27 (122) :17-22
URL: http://journal.zums.ac.ir/article-1-5294-en.html
A Study on the Effects of Perovskia abrotanoides Karel on Experimental Skin Burn in Male Rat: in-vivo and in-vitro Findings
Amin Derakhshanfar1 , Seyedeh-Sara Hashemi *2 , Javad Moayedi3 , Mahjoob Vahedi4 , Davood Mehrabani5 , Abuzar Valizadeh4
1- Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2- Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran , sara_hashemi@sums.ac.ir
3- Burn and Wound Healing Research Center, Shiraz University of Medical Science, Shiraz, Iran
4- Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5- Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz,Iran
Abstract:   (148131 Views)

Background & Objective: Perovskia abrotanoides Karel locally known as Brazambal, is widely used in medical practice to treat various disorders. Burn is a common source of injury and Brazambal ointment has been extensively used in burn wound healing in North of Iran. The purpose of this study was to examine the healing effect of Brazambal ointment in experimental model of burn. In addition, in-vitro healing characteristics and antibacterial activity of P. abrotanoides was investigated.
Materials & Methods: Scald burn injury was produced by pouring 2.5cc of boiling water (95ºC) on the posterior surface of rat’s neck for 10 seconds. Twenty-eight adult male Sprague Dawley rats were divided into 4 groups (7 rats each) and followed for 7 days. Control group (no treatment); Silver sulfadiazine-treated group; Brazambal ointment-treated group; and vehicle-treated group received the complete ointment without Brazambal part. The effects of Brazambal aqueous extract on fibroblast cells and antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were evaluated.
Results: Topical application of Brazambal ointment can accelerate wound healing process by initiating re-epithelialization and formation of granulation tissue. Proliferative activity of the aqueous extract on fibroblast cells was observed after 48 h in concentrations of 7.5% and 10% (P<0.0001). However, P. abrotanoides Karel extract had no significant antibacterial effect.
Conclusion: The presence of hair follicles, re-epithelialization and granulation tissue is suggestive of Brazambal ointment relative effectiveness on experimental scald burn in rats. However, further studies are warranted to reveal the healing mechanisms of P. abrotanoides.

Keywords: Perovskia abrotanoides, Brazambal, Traditional medicine, Burn
Full-Text [PDF 686 kb]   (157693 Downloads) |   |   Full-Text (HTML)  (3891 Views)  

✅ The presence of hair follicles, re-epithelialization and granulation tissue is suggestive of Brazambal ointment relative effectiveness on experimental scald burn in rats. However, further studies are warranted to reveal the healing mechanisms of P. abrotanoides.


Type of Study: Original Research Article | Subject: Clinical Medicine
Received: 2018/08/29 | Accepted: 2019/03/28 | Published: 2019/05/10
References
1. Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R. The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. Int J Clin Med. 2015; 6(9): 635-42. [DOI:10.4236/ijcm.2015.69084]
2. Atanasov AG, Waltenberger B, Pferschy-Wenzig E-M, Linder T, Wawrosch C, Uhrin P,et al. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv. 2015; 33(8): 1582-614. [DOI:10.1016/j.biotechadv.2015.08.001] [PMID] [PMCID]
3. Ghasemi Pirbalouti A, Koohpayeh A, Karimi I. The wound healing activity of flower extracts of Punica granatum and Achillea kellalensis in Wistar rats. Acta Pol Pharm. 2010; 67(1): 107-10.
4. Shahraki S, Mahdavi S, Hosseini S, Mazandarani M, Tavan M. Investigation quantity and quality of essential oils the Proveskia abrotanoides karel (case study: International park of golestan and kyasar mazandaran). ECO-Phytochem J Med Plant. 2013; 1(3): 68-81.
5. Beikmohammadi M. The evaluation of medicinal properties of Perovskia abrotanoides Karel. Middle-East J Sci Res. 2012; 11(2): 189-93.
6. Kolbady Nejad S, Jaimand K, Monfared A, Akbarzadeh M. Study of the chemical composition of essential oils of Perovskia abrotanoides karel at the different stage and distillation by gas chromatography. JMPB. 2013; 2(2): 139-42.
7. Mahboubi M, Kazempour N. The antimicrobial activity of essential oil from Perovskia abrotanoides karel and its main components. Indian J Pharm Sci. 2009; 71(3): 343-7. [DOI:10.4103/0250-474X.56016] [PMID] [PMCID]
8. Mazandarani M, Ghaemi E. Ethnopharmacological investigation of different parts of Perovskia abrotanoides Karel. Planta Med. 2010; 76(12): 123. [DOI:10.1055/s-0030-1264421]
9. Morteza-Semnani K. The essential oil composition of Perovskia abrotanoides from Iran. Pharm Biol. 2004; 42(3): 214-6. [DOI:10.1080/13880200490514078]
10. Sajjadi S, Mehregan I, Khatamsaz M, Asgari G. Chemical composition of the essential oil of Perovskia abrotanoides Karel growing wild in Iran. Flavour Fragr J. 2005; 20(4): 445-6. [DOI:10.1002/ffj.1508]
11. Moallem SA, Niapour M. Study of embryotoxicity of Perovskia abrotanoides, an adulterant in folk-medicine, during organogenesis in mice. J Ethnopharmacol. 2008; 117(1): 108-14. [DOI:10.1016/j.jep.2008.01.020] [PMID]
12. Ashraf SN, Zubair M, Rizwan K, Tareen RB, Rasool N, Zia-Ul-Haq M, et al. Compositional studies and biological activities of Perovskia abrotanoides Karel oils. Biol Res. 2014; 47(1): 12-20.
13. Hosseinzadeh H, Amel S. Antinociceptive effects of the aerial parts of Perovskia abrotanoides extracts in mice. Iran Red Crescent Med J. 2001; 4(1): 15-7.
14. Nasiriasl M, Parvardeh S, Niapour M, Hosseinzadeh H. Antinociceptive and anti-inflammatory effects of Perovskia abrotanoides aerial part extracts in mice and rats. J Med Plants. 2002; 1(3): 1-8.
15. Sairafianpour M, Christensen J, Stærk D, Budnik B, Kharazmi A, Bagherzadeh K, et al. Leishmanicidal, antiplasmodial, and cytotoxic activity of novel diterpenoid 1, 2-quinones from Perovskia abrotanoides: new source of tanshinones. J Nat Prod. 2001; 64(11): 1398-403. [DOI:10.1021/np010032f] [PMID]
16. Jaafari MR, Hooshmand S, Samiei A, Hossainzadeh H. Evaluation of-leishmanicidal effect of Perovskia abrotanoides Karel root extract by in vitro leishmanicidal assay using promastigotes of Leishmania major. Pharmacol Online. 2007; 1(1): 299-303.
17. Hashemi S-S, Mahmoodi M, Rafati AR, Manafi F, Mehrabani D. The role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration of human skin fibroblasts. World J Plast Surg. 2017; 6(2): 198-205.
18. Hashemi S, Rafati A. Comparison between human cord blood serum and platelet-rich plasma supplementation for human wharton's jelly stem cells and dermal fibroblasts culture.IJMRHS. 2016; 5(8): 191-6
19. Pereira T, Dos Santos D, Lima-Ribeiro MHM, de Pontes-Filho NT, Carneiro-Leão AMdA, Correia MTdS. Development of animal model for studying deep second-degree thermal burns. J Biomed Biotechnol. 2012; 2012(1): 1-7. [DOI:10.1155/2012/460841] [PMID] [PMCID]
20. Abdullahi A, Amini-Nik S, Jeschke M. Animal models in burn research. Cell Mol Life Sci. 2014; 71(17): 3241-55. [DOI:10.1007/s00018-014-1612-5] [PMID] [PMCID]
21. Qu M, Nourbakhsh M. Current experimental models of burns. Discov Med. 2017; 23(125): 95-103.
22. Campelo APBS, Campelo MWS, Britto GAdC, Ayala AP, Guimarães SB, Vasconcelos PRLd. An optimized animal model for partial and total skin thickness burns studies. Acta Cir Bras. 2011; 26(1): 38-42. [DOI:10.1590/S0102-86502011000700008] [PMID]
23. Abdeldjelil M, Messai A, Boudebza A, Beghoul S. Practical aspects to generate cutaneous experimental burns in a rat model. Pharm Lett. 2017; 9(1): 70-84.
24. Guo H-F, Ali RM, Hamid RA, Zaini AA, Khaza'ai H. A new model for studying deep partial-thickness burns in rats. Int J Burns Trauma. 2017; 7(6): 107-14.
25. Geryani MA, Mahdian D, Mousavi SH, Hosseini A. Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines. Avic J Phy. 2016; 6(4): 410-7.
26. Nezhadali A, Masrornia M, Solati A, Akbarpour M, Nakai Moghaddam M. Analysis of the flower essential oil at different stages of plant growth and in vitro antibacterial activity of Perovskia abrotanoides Karal in Iran. Der Pharma Chemica. 2009; 1(1): 146-50.
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb