Volume 31, Issue 145 (March & April 2023)                   J Adv Med Biomed Res 2023, 31(145): 191-196 | Back to browse issues page

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Wibisono D S, Sucahyo J F, Fulyani F, Ariani M D, Nugroho E A, Daniswara N. Effect of Temulawak (Curcuma xanthorrhiza) Extract on Sperm Counts, Morphology, and Motility of Nicotine-Induced Mice. J Adv Med Biomed Res 2023; 31 (145) :191-196
URL: http://journal.zums.ac.ir/article-1-6838-en.html
Effect of Temulawak (Curcuma xanthorrhiza) Extract on Sperm Counts, Morphology, and Motility of Nicotine-Induced Mice
Dimas Sindhu Wibisono * 1, Josephine Fiona Sucahyo2 , Faizah Fulyani3 , Mahayu Dewi Ariani3 , Eriawan Agung Nugroho4 , Nanda Daniswara4
1- Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia , uroandrology21@gmail.com
2- Faculty of Medicine, Diponegoro University, Semarang, Indonesia
3- Dept. of Medical Biology and Biochemistry, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
4- Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Abstract:   (10731 Views)

Background and Objective: Infertility is a health problem that affects 72.4 million couples worldwide. Infertility can occur as a result of an unhealthy lifestyle, such as a smoking habit. Cigarettes contain nicotine, a toxic compound that can trigger oxidative stress and eventually decrease fertility. Temulawak is a medicinal plant that is rich in antioxidants and anti-inflammatory properties. It has been used as a traditional remedy and is believed to improve fertility. We aim to study the effect of temulawak extract on the morphology, motility, and sperm counts of nicotine-induced mice.
Materials and Methods: A total of 20 mice were divided into four groups (n = 5): control normal (K0); nicotine-induced (K1); and nicotine-induced treated with two different concentrations of temulawak extract, 4 mg/20 g body weight (P1) and 8 mg/20 g body weight (P2). The mice were given nicotine and temulawak for 28 days and then killed by neck dislocation. Both testes and vas deferens were collected for sperm analysis.
Results: Mice treated with nicotine (K1) showed lower sperm counts (p=0.012) and motility (p<0.001) compared to mice in the control normal (K0) but had no difference in morphology. Treatment with temulawak (4 mg/20 g body weight) on nicotine-induced mice (P1) significantly increased sperm motility (p< 0.001). A double dose of temulawak extract (P2) resulted in significantly higher motility and sperm counts than in group K1.
Conclusion: Temulawak treatment (8 mg/20 g body weight) on nicotine-induced mice can significantly improve motility and sperm counts.

Keywords: Nicotine, Temulawak, Spermatozoa, Infertility
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✅Temulawak treatment (8 mg/20 g body weight) on nicotine-induced mice can significantly improve motility and sperm counts.


Type of Study: Original Article | Subject: Life science
Received: 2022/01/21 | Accepted: 2022/09/15 | Published: 2023/03/13
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