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J Adv Med Biomed Res 2024, 32(152): 226-234 |
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Khavasi N, Tavakolizadeh M, sardari S, ghassemifard L, reshadatifar M, darkhori M, et al . Evaluation of Antifungal and Antibacterial Effects of Capparis spinosa Root Extract on Streptococcus Mutans and Candida albicans. J Adv Med Biomed Res 2024; 32 (152) :226-234
URL: http://journal.zums.ac.ir/article-1-7357-en.html
URL: http://journal.zums.ac.ir/article-1-7357-en.html
Narjes Khavasi1 
, Mahdi Tavakolizadeh2 , Saeed Sardari1 , Leila Ghassemifard1 , Mina Reshadatifar3 , Milad Darkhori1 , Mina Mohebian * 4, Fakhri Haghi5 , Saeid Amanloo6 , Kamyar Mansori7
, Mahdi Tavakolizadeh2 , Saeed Sardari1 , Leila Ghassemifard1 , Mina Reshadatifar3 , Milad Darkhori1 , Mina Mohebian * 4, Fakhri Haghi5 , Saeid Amanloo6 , Kamyar Mansori7
1- Department of Persian Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran 3. Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of
3- Department of Oral and maxilla facial medicine, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
4- Department of Oral and maxilla facial medicine, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran ,m.mohebian@zums.ac.ir
5- Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
6- Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
7- Social Determinants of Health Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
2- Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran 3. Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of
3- Department of Oral and maxilla facial medicine, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
4- Department of Oral and maxilla facial medicine, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran ,
5- Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
6- Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
7- Social Determinants of Health Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract: (157 Views)
Background & Objective: Capparis spinosa is a plant belonging to the genus Capari which is important for medicinal purposes. It is also known as a multipurpose plant in Persian medicine. In this study, we searched for natural active ingredients in mouthwash for immunocompromised patients and examined the antifungal plus antibacterial properties of the hydroalcoholic extract of the root of the Capparis plant against Streptococcus mutans (S. mutans) PTCC 1683 and Candida albicans (C. albicans) ATCC-10231 was investigated.
Materials & Methods: Ethanol extracts of plant roots were extracted by the percolation method. The diameter of the growth inhibition zone of C. albicans and S. mutans extracts was measured using the well plate method. Minimum inhibitory concentrations (MICs) were calculated for bacterial and fungal strains. In addition, the minimum sterilization concentration (MBC) and minimum sterilization concentration (MFC) of the extract were investigated using the broth microdilution method. The results were compared with an antibacterial and antifungal drug of 0.2% chlorhexidine nystatin.
Results: The results for both pathogens revealed that the diameter of the growth inhibition zone increased with elevation of the extract concentration. The MIC and MBC of S. mutans extract were 62.5 and 62.5 mg/ml, compared to 0.39 and 0.39 mg/ml for chlorhexidine. The MIC and MFC of the plant against the fungus C. albicans were 7.812 and 31.25 mg/ml, compared to 0.25 and 1.25 μg/ml for nystatin.
Conclusion: The ethanol extract of the Caparis plant had an antifungal effect on C. albicans and an antibacterial effect on S. mutans. However, the inhibitory and lethal effects against bacterial and fungal strains were lower than those of nystatin and chlorhexidine.
Materials & Methods: Ethanol extracts of plant roots were extracted by the percolation method. The diameter of the growth inhibition zone of C. albicans and S. mutans extracts was measured using the well plate method. Minimum inhibitory concentrations (MICs) were calculated for bacterial and fungal strains. In addition, the minimum sterilization concentration (MBC) and minimum sterilization concentration (MFC) of the extract were investigated using the broth microdilution method. The results were compared with an antibacterial and antifungal drug of 0.2% chlorhexidine nystatin.
Results: The results for both pathogens revealed that the diameter of the growth inhibition zone increased with elevation of the extract concentration. The MIC and MBC of S. mutans extract were 62.5 and 62.5 mg/ml, compared to 0.39 and 0.39 mg/ml for chlorhexidine. The MIC and MFC of the plant against the fungus C. albicans were 7.812 and 31.25 mg/ml, compared to 0.25 and 1.25 μg/ml for nystatin.
Conclusion: The ethanol extract of the Caparis plant had an antifungal effect on C. albicans and an antibacterial effect on S. mutans. However, the inhibitory and lethal effects against bacterial and fungal strains were lower than those of nystatin and chlorhexidine.
Keywords: Persian Medicine, Mouthwash, Immunosuppress, Antifungal agents, Antimicrobial agents, Candida albicans Capparis spinosa, Streptococcus mutans
Type of Study: Original Article |
Subject:
Medical Biology
Received: 2023/09/28 | Accepted: 2024/09/10 | Published: 2024/06/21
Received: 2023/09/28 | Accepted: 2024/09/10 | Published: 2024/06/21
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