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Volume 33, Issue 157 (March & April 2025)
J Adv Med Biomed Res 2025, 33(157): 5-5 |
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Ethics code: IR.KUMS.MED.REC.1401.04 and IR.KUMS.MED.REC.1401.209
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Khani-Hematabadi F, Rashidi I, Ghanbari A, Pazhouhi M, Zhaleh M, Rostami E et al . Anti-Metastatic Potential of Hydroalcoholic Extract of Pistacia Khinjuk Leaves and Its Mechanism: An in Vitro Study. J Adv Med Biomed Res 2025; 33 (157) :5-5
URL: http://journal.zums.ac.ir/article-1-7495-en.html
URL: http://journal.zums.ac.ir/article-1-7495-en.html
Fuzieh Khani-Hematabadi1 
, Iraj Rashidi1 , Ali Ghanbari1 , Mona Pazhouhi1 , Mohsen Zhaleh *2 , Erfan Rostami3 , Reza Taravideh3
, Iraj Rashidi1 , Ali Ghanbari1 , Mona Pazhouhi1 , Mohsen Zhaleh *2 , Erfan Rostami3 , Reza Taravideh3
1- Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ,zhaleh.mohsen@gmail.com
3- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ,
3- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract: (25 Views)
Background and Aims: Metastasis represents the primary contributor to mortality associated with cancer. Traditionally, Pistacia khinjuk has been utilized for addressing various ailments. Considering the reported anticancer effects of Pistacia Khinjuk, this research seeks to explore the anti-metastatic properties of the hydroalcoholic extract derived from this indigenous Iranian herb on prostate and breast cancer cells.
Materials and Method: Hydroalcoholic extract was prepared, and its cytotoxicity was tested. The extract's impact on the migration, invasion, and adhesion capabilities was analyzed. Matrix metalloproteinases-2 and -9 secretions were evaluated. Also, metastatic-related gene expression levels were evaluated.
Results: After 24, 48, 72, and 96 hrs, the extract decreased the cancer cells viability in a concentration- and time-dependent manner. Notably, breast cancer cells demonstrated greater sensitivity to the extract's cytotoxic effects than prostate cancer cells. After 24 hrs of treatment with half-maximal inhibitory concentration, there was a significant reduction in the cell migration, invasion, and adhesion capabilities (p<0.05). Additionally, matrix metalloproteinases-2 and -9 levels were reduced. Also, the extract significantly decreased matrix metalloproteinases-2 and -9, urokinase, and urokinase receptorg genes expression (p<0.05). Conversely, tissue inhibitors of metalloproteinase-1 and -2 gene expression levels significantly increased (p<0.05).
Conclusion: Altogether, Pistacia khinjuk leaves hydroalcoholic extract had cytotoxic effects on breast and prostate cancer cells and reduced the metastasis potential.
Type of Study: Original Research Article |
Subject:
Medical Biology
Received: 2024/12/12 | Accepted: 2025/04/22 | Published: 2025/05/29
Received: 2024/12/12 | Accepted: 2025/04/22 | Published: 2025/05/29
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