Mon, May 18, 2026
[Archive]
Articles In Press
Back to the articles list |
Back to browse issues page
1- Department of Microbial Biotechnology, College of Biotechnology AL-Nahrain University, Jadriga, Baghdad, Iraq , dina.naseer@nahrainuniv.edu.iq
2- Chemistry Department, College of Science, Al-Nahrain University, Baghdad, Iraq
2- Chemistry Department, College of Science, Al-Nahrain University, Baghdad, Iraq
Abstract: (6 Views)
Background and Objective: Pancreatic cancer is one of the most aggressive cancers, requiring innovative chemotherapeutic treatments with increased selectivity and effectiveness.
Methods: A new heterocyclic derivatives of dihydroquinazolin, oxazepine, tetrazole, and thiazine through the Schiff base derivative D were synthesized. This research used FTIR and ^1H-NMR spectroscopy to study Schiff base-derived heterocyclic compounds having dihydroquinazolin, oxazepine, tetrazole, and thiazine moieties. To evaluate selectivity, the synthesised compounds were tested in vitro against PANC-1 and HDFn cells.
Results: The compounds inhibited proliferation dose-dependently in MTT assays. Derivative D4 exhibited greater selectivity for cancer cells, (IC₅₀ = 129.5 µg/mL for PANC-1 and 237.4 µg/mL for HDFn cells), whereas derivative D5 exhibited stronger but less selective cytotoxicity (IC₅₀ = 68.9 and 61.01 µg/mL for PANC-1 and HDFn cells Due to increased nuclear intensity and membrane permeability, high-content screening of D4 decreased viable cell count and mitochondrial membrane potential. Caspase-8 and 9 activity significantly enhanced at 100 and 200 µg/mL (P = 0.0019 and P < 0.0001), indicating intrinsic and extrinsic apoptosis induction.
Conclusion: These findings suggest that Schiff base–derived heterocycle structural alteration optimization for tumor selectivity and biological safety may provide anticancer benefits.
Methods: A new heterocyclic derivatives of dihydroquinazolin, oxazepine, tetrazole, and thiazine through the Schiff base derivative D were synthesized. This research used FTIR and ^1H-NMR spectroscopy to study Schiff base-derived heterocyclic compounds having dihydroquinazolin, oxazepine, tetrazole, and thiazine moieties. To evaluate selectivity, the synthesised compounds were tested in vitro against PANC-1 and HDFn cells.
Results: The compounds inhibited proliferation dose-dependently in MTT assays. Derivative D4 exhibited greater selectivity for cancer cells, (IC₅₀ = 129.5 µg/mL for PANC-1 and 237.4 µg/mL for HDFn cells), whereas derivative D5 exhibited stronger but less selective cytotoxicity (IC₅₀ = 68.9 and 61.01 µg/mL for PANC-1 and HDFn cells Due to increased nuclear intensity and membrane permeability, high-content screening of D4 decreased viable cell count and mitochondrial membrane potential. Caspase-8 and 9 activity significantly enhanced at 100 and 200 µg/mL (P = 0.0019 and P < 0.0001), indicating intrinsic and extrinsic apoptosis induction.
Conclusion: These findings suggest that Schiff base–derived heterocycle structural alteration optimization for tumor selectivity and biological safety may provide anticancer benefits.
Keywords: Pancreatic Neoplasms, Cytotoxicity, Schiff Bases, Heterocyclic Compounds, Apoptosis, Caspase Activation
Type of Study: Original Research Article |
Subject:
Life Science
Received: 2025/12/8 | Accepted: 2026/05/11
Received: 2025/12/8 | Accepted: 2026/05/11
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Journal of Advances in Medical and Biomedical Research by Farname Inc. is licensed under CC BY-NC 4.0
