Volume 25, Issue 112 (7-2017)                   J Adv Med Biomed Res 2017, 25(112): 40-49 | Back to browse issues page

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Ghotaslou A, Boustani H, Rostami E, Ghalesardi O, Sadeghian M H. Evaluation of GRAF Gene Expression in Patients with B-ALL Referred to Mashhad Hospitals and Its Relationship with Clinical and Laboratory Findings. J Adv Med Biomed Res 2017; 25 (112) :40-49
URL: http://journal.zums.ac.ir/article-1-4589-en.html
1- Student Research Committee, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
2- Dept. of Laboratory Sciences, Faculty of Para- Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
3- Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:   (151277 Views)

Background and Objective: Acute lymphoblastic leukemia is a disorder caused by the proliferation of malignant immature lymphoid cells. GRAF (GTPase regulator associated with focal adhesion kinase) which acts as a tumor suppressor gene is disabled in hematologic malignancies due to genetic and epigenetic abnormalities. In this study, GRAF gene expression was inspected in patients with B-ALL.

Materials and Methods: Peripheral blood samples from 60 patients with B-ALL and 30 healthy controls were collected. DNA was extracted and cDNA was synthesized. GRAF gene expression was measured using Real-Time PCR and the relationship between GRAF expression and clinical and laboratory findings were investigated.

Results: GRAF gene expression was significantly decreased in patients compared to the control group (0.87).
The number of patients who had a reduction in their GRAF gene expression was 73.4% (44 of 60 patients). GRAF expression had no significant difference in the FAB morphological subtypes.

Conclusion: Results of this study showed that GRAF gene expression is decreased in acute lymphoblastic leukemia. The reduction in GRAF gene expression confirms its tumor suppressive role in this leukemia.

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Type of Study: Clinical Trials |
Received: 2017/06/14 | Accepted: 2017/06/14 | Published: 2017/06/14

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