Volume 33, Issue 162 (Special Issue 2025)                   J Adv Med Biomed Res 2025, 33(162): 61-67 | Back to browse issues page

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Hasan S A, Al Rikaby A A. Assessment of the Protective Effect of Rosemary Leaves Extract Against Oxidative Stress and Inflammatory Cytokines Induced by Cypermethrin in Male Rabbits. J Adv Med Biomed Res 2025; 33 (162) :61-67
URL: http://journal.zums.ac.ir/article-1-7865-en.html
Assessment of the Protective Effect of Rosemary Leaves Extract Against Oxidative Stress and Inflammatory Cytokines Induced by Cypermethrin in Male Rabbits
Shireen Ali Hasan *1 , Ahlam A. Al Rikaby2
1- Department of Pharmacology and Toxicology, College of Pharmacy, University of Thi-Qar, Thi-Qar, 64001, Iraq , shireenalihasan@utq.edu.iq
2- Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
Abstract:   (132 Views)

Background & Objective: Rosmarinus officinalis (rosemary) is widely used in the daily diet and has recently gained increasing attention for its medicinal and pharmacological properties, particularly its antioxidant and anti-inflammatory potential. Cypermethrin (CYP) is a synthetic pyrethroid insecticide that induces oxidative stress and inflammation in biological systems. This study aimed to evaluate the protective efficacy of rosemary leaf extract against cypermethrin-induced oxidative stress, inflammatory response, and neurotoxicity in male rabbits.
 Materials & Methods: Forty male rabbits were randomly divided into four groups (n=10 each).Group 1 (control): received 1 mL of corn oil. Group 2 (CYP): orally administered CYP at 66.5 mg/kg b.w (1/10 LD50).Group 3: received rosemary extract (100 mg/kg) + CYP (66.5 mg/kg).Group 4: received rosemary extract (200 mg/kg) + CYP (66.5 mg/kg). All treatments were administered orally once daily for five weeks. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), inflammatory cytokines (TNF-α and IL-6), and acetylcholinesterase (AChE) activity were measured.
Results:  CYP exposure significantly increased MDA, TNF-α, and IL-6 levels and decreased TAC and AChE activity compared to the control group, indicating oxidative stress, inflammatory response, and neurotoxicity. Co-administration of rosemary extract with CYP resulted in a significant reduction in MDA and inflammatory cytokines, along with a marked increase in TAC and AChE levels compared to the CYP-only group.
Conclusion:  The findings demonstrate that Rosmarinus officinalis possesses potent antioxidant, anti-inflammatory, and neuroprotective properties. Rosemary extract effectively mitigated cypermethrin-induced oxidative damage, inflammation, and cholinesterase inhibition in male rabbits.

Keywords: Rosmarinus Officinalis, Cypermethrin, Oxidative Stress, TNF-α, IL-6, TAC, AChE
Full-Text [PDF 473 kb]   (7 Downloads)    
Type of Study: Original Research Article | Subject: Medical Biology
Received: 2025/09/29 | Accepted: 2025/11/19 | Published: 2025/12/29
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